Product Description
EUDirect PCR kit facilitates PCR amplification directly from the small amount of samples without DNA extraction and purification. It allows direct PCR amplification from a variety of samples including whole blood, blood collected on card, saliva, bacteria, mouse tail, tissue, cultured cells or plant.
Components
|
Catalog |
Description |
Quantity |
Storage |
| EUD12S | EUDirect 2X PCR Master Mix |
4 X 1.5 mL |
-20°C |
| EUD12LS | EUDirect Lysis Buffer |
25 mL |
RT |
| EUD12E | EUDirect Enzyme Mix |
0.5 mL |
-20°C |
| EUD12EC | Eukaryote Control Primer Mix |
100 μl |
-20°C |
| EUD12PC | Prokaryote Control Primer Mix |
100 μl |
-20°C |
Lysis protocol
1. Add suggested amount of starting material to 1.5 mL eppendorf tube
– Bacteria/mammalian cells: 10 – 20 µl of cells
– Mouse and other animals: 0.1 – 0.2 cm sample (using a puncher or scalpel)
– Plant and seed: 0.1 – 0.2 cm sample (using a puncher or scalpel)
– Blood: 2 – 10 µl of whole blood
2. Add 100 µl of Lysis Buffer and 2 µl of Enzyme Mix to each tube, vortex briefly
3. Incubate the tube at 65 ℃ for 20 minutes, then transfer to 95 ℃ for 5 minutes
4. Use 1 – 2 µl of lysates for PCR reactions
Universal control primer mix
The kit includes two primer mixes to use as the positive control:
Eukaryote Control Primer Mix: Contains primers designed to amplify the specific region (target size 700 bps) of eukaryotic 18s DNA. This should be used when performing direct PCR with blood, mammalian cells, mouse, animal or plant.
Prokaryote Control Primer Mix: Contains primers designed to amplify the specific region (target size 150 bps) of prokaryotic 16s DNA. This should be used when performing direct PCR with bacteria cells.
Shipping and Storage
We ship at room temperature to reduce the shipping cost. The product is stable at 37°C for several weeks and can be stored at 4°C for several months. For long-term storage, please keep it at -20°C.
Manual
https://eu-bio.com/EUDirect-PCR-Kit.pdf