Custome Molecular Cloning
Cloning can sometimes be tedious and time-consuming. Let EU-BIO do the job. We will take care of your project and perform the following steps:
- Consulting
Comprehensive support in the selection of an appropriate cloning strategy - Experimental performance of the specific DNA amplification
E.g. amplification of complete mRNA sequences by applying optimized RT-PCR technologies - Gene synthesis
Using our technology, we successfully synthesize DNA fragments and complete genes up to several kb - Molecular cloning
Cloning of full-length cDNAs, synthetic genes, PCR products or vector inserts using standard or more sophisticated molecular cloning techniques - Quality control
Each vector construct generated at Eurofins Genomics undergoes strict quality control. We perform differential analyses by restriction enzyme digest as well as DNA sequencing to identify correct clones and to verify sequence accuracy. - Delivery and documentation
The cloned fragments are delivered as E. coli clones or plasmid DNA (mini scale preparation). Higher scales are available on request. The experimental strategy, raw data and final results are summarised in a detailed report.
Site-Directed Mutagenesis
We use a targeted PCR-based process to introduce genetic variation precisely, and then clone the construct into the vector of your choice. EU-BIO’s site-directed mutagenesis services include point mutations, deletions, and insertions. Our services can provide you a fast, cost-saving way to get 100% accurate mutant DNA clones.